Exponential megapriming PCR (EMP) cloning--seamless DNA insertion into any target plasmid without sequence constraints.

Exponential megapriming PCR (EMP) cloning--seamless DNA insertion into any target plasmid without sequence constraints.

Blog Article

We present 5326058hx a fast, reliable and inexpensive restriction-free cloning method for seamless DNA insertion into any plasmid without sequence limitation.Exponential megapriming PCR (EMP) cloning requires two consecutive PCR steps and can be carried out in one day.We show that EMP cloning has a higher efficiency than restriction-free (RF) cloning, especially for long sequal eclipse 5 battery inserts above 2.

5 kb.EMP further enables simultaneous cloning of multiple inserts.